Europe captain Paul McGinley has selected Graeme McDowell to open up his side's bid to retain the Ryder Cup in Sunday's singles action.
The Northern Irishman, who has partnered Victor Dubuisson to two points on Friday and Saturday, will play American rookie Jordan Spieth.
Dubuisson will tee off last against Zach Johnson as the hosts look for the four points that they require to retain the trophy that they won in Medinah two years ago.
Henrik Stenson is out second against Patrick Reed, Rory McIlroy follows against Rickie Fowler while form player Justin Rose will play Hunter Mahan.
The other stand-out tie sees home favourite Stephen Gallacher face Phil Mickelson.
Full lineup
Graeme McDowell vs. Jordan Spieth
Henrik Stenson vs. Patrick Reed
Rory McIlroy vs. Rickie Fowler
Justin Rose vs. Hunter Mahan
Stephen Gallacher vs. Phil Mickelson
Martin Kaymer vs. Bubba Watson
Thomas Bjorn vs. Matt Kuchar
Sergio Garcia vs. Jim Furyk
Ian Poulter vs. Webb Simpson
Jamie Donaldson vs. Keegan Bradley
Lee Westwood vs. Jimmy Walker
Victor Dubuisson vs. Zach Johnson