Tranmere Rovers have suspended manager Ronnie Moore while the Football Association determines whether he has breached betting rules.
On Friday, it emerged that the FA wanted to speak to Moore, 61, over claims he broke rules by betting in a competition Tranmere are involved in.
Moore took charge of Saturday's 2-2 draw against Bristol City, but today he was suspended.
In a statement, the club said: "Tranmere Rovers have today announced that with immediate effect manager Ronnie Moore has been suspended until the conclusion of a Football Association investigation into a potential breach of betting rules.
"Assistant manager John McMahon has been placed in temporary charge of first team affairs. The club will be making no further comment until the outcome of the investigation."
Tranmere currently lie 18th in League One, two points above the relegation zone.