Benfica are unchanged from the side which last week demolished fellow Primeira Liga side Belenenses 6-0 for their Champions League tie against Astana on Tuesday.
Jonas and Konstantinos Mitroglou continue their strike partnership after each contributing two goals in Friday's league game, while Nicolas Gaitan and Talisca will also be looking to add to their goal tally.
Meanwhile, Astana will become the first Kazakh side to play a Champions League group match when the first whistle blows at the Estadio da Luz.
Manager Stanimir Stoilov has made a number of changes to his side from last week's league game against Ordabasy, with top scorers Tanat Nuserbayev and Nemanja Maksimovic both missing out.
Benfica: Júlio Cesar, Nelson Semedo, Luisao, Jardel, Eliseu, Samaris, Talisca, Goncalo Guedes, Gaitan, Jonas, Mitroglou
Subs: Moraes, Fejsa, Jimenez, Pizzi, Silvio, Andrade, Nuno Santos
Astana: Eric; Ilic, Postnikov, Anicic, Shomko; Canas, Maksimovic; Dzholchiyev, Zukhov, Kethevoama; Kabananga
Subs: Akhmetov, Shchetkin, Muzhikov, Pikalkin, Beysebekov, Dedechko, Golubnichy
