James Collins and Jack Collison have been forced to withdraw from the Wales squad to face Bosnia-Herzegovina.
Collins has been ruled out with a groin injury, while Collison will miss the match due to a knee injury.
Huddersfield Town's Joel Lynch and Cardiff City's Robert Earnshaw have been called up as replacements.
The match represents Chris Coleman's final opportunity to run the rule over his squad before they begin their World Cup qualifying matches in September.