The Football Association have rejected Sunderland's appeal against the red card that Stephane Sessegnon picked up against Aston Villa on Monday.
The 28-year-old was given his marching orders by referee Lee Probert for a late challenge on Yacouba Sylla during Villa's 6-1 win at Villa Park.
The Black Cats launched an appeal on Tuesday, but the FA have revealed that they have rejected the appeal.
The statement said: "At an Independent Regulatory Commission hearing today, Stephane Sessegnon's claim of wrongful dismissal was unsuccessful meaning the Sunderland midfielder's three-match suspension remains with immediate effect.
"Sessegnon was dismissed for serious foul play during his side's game against Aston Villa on Monday April 29, 2013."
As a result, the midfielder will now miss the remainder of the season.